Response: Nitroblue tetrazolium (NBT) assay
نویسندگان
چکیده
منابع مشابه
The nitroblue tetrazolium (NBT) test in renal transplantation.
The nitroblue tetrazolium (NBT) test has been evaluated as a means of differentiating episodes of infection and rejection in 10 cadaver kidney transplant recipients. Normal NBT values were observed during all 11 episodes of acute rejection. A significant elevation of the NBT score was encountered in six of eight episodes of infection after transplantation. A positive NBT result is useful additi...
متن کاملThe mechanism of the entry of dye into neutrophils in the nitroblue tetrazolium (NBT) test.
1 . Washed buffy-coat preparations of human blood suspended in different media were exposed to the Nitroblue Tetrazolium (NBT) and the mechanism of entry of the dye into neutrophil polymorphonuclear leucocytes was investigated. 2. Stimulation of neutrophils with endotoxin and the presence of heparin and/or fibrinogen in the suspending medium were necessary for dye reduction. 3. NBT complexed wi...
متن کاملThe spontaneous and stimulated nitroblue tetrazolium (NBT) tests in mononuclear cells of patients with tuberculosis.
PURPOSE The aim of this study was to evaluate the ability of the NBT reduction by non- and BCG-stimulated monocytes isolated from peripheral blood persons with pulmonary tuberculosis (TB) before treatment, after two-month antituberculosis therapy and in an inactive stage of this disease. MATERIAL AND METHODS The spontaneous and induced NBT tests were done in 24 healthy individuals and 59 pati...
متن کاملA convenient assay of glycoserum by nitroblue tetrazolium with iodoacetamide.
BACKGROUND To determine glycoserum, the nitroblue tetrazolium (NBT) assay is quick, economical, convenient and easily automated. This method, however, is vulnerable to interference by thiol group, which should not be ignored during the analysis. METHODS Thiol group in glycoserum was blocked with iodoacetamide (IAM) before NBT was added. The reaction was carried out in a thermal bath and termi...
متن کاملNitroblue tetrazolium test in experimental syphilis.
The reduction of nitroblue tetrazolium (NBT) by phagocytic leucocytes in peripheral blood has been examined in rabbits injected intratesticularly with Treponema pallidum, T. pallidum-free supernatant of orchitic testes, or normal rabbit testes supernatant. A significant (P less than 0.01) increase in NBT reduction was observed only in the infected animals.
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2018
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2017.09.007